The objective of this workshop is for you to walk away with a thorough understanding of how to correctly conduct qPCR design and analysis.

- Properly understand the initial stage of RNA to cDNA conversion;

- Truly understand the Analysis principles;

- Comprehend the different Analysis levels;

- Show why there is bias in estimated efficiency;

- Demonstrate how bias is introduced by the delta-delta Cq method;

- Show how to avoid bias by using LinRegPCR;

- See how to correctly run multiplate experiments.