Design and Analysis of qPCR experiments: how to reach unbiased reports

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Genome Institute

60 Biopolis Street

Singapore, 138672

Singapore

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Learn the most up to date qPCR analysis methods from one of the world’s leaders in qPCR, Dr Jan Ruijter

About this Event

The objective of this workshop is for you to walk away with a thorough understanding of how to correctly conduct qPCR design and analysis.

- Properly understand the initial stage of RNA to cDNA conversion;

- Truly understand the Analysis principles;

- Comprehend the different Analysis levels;

- Show why there is bias in estimated efficiency;

- Demonstrate how bias is introduced by the delta-delta Cq method;

- Show how to avoid bias by using LinRegPCR;

- See how to correctly run multiplate experiments.

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Genome Institute

60 Biopolis Street

Singapore, 138672

Singapore

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